Antimicrobial activity assays: Microdilution, killing kinetics, motility assays, determination of virulence factor activities (e.g., pyocyanin production)

Antibiofilm activity assays: Crystal violet assay, viability assays, and microscopic analyses

Peptide characterization: FT-IR 

Investigation of biofilm resistance mechanisms: RT-PCR analyses, pull-down assay, proteomic approaches

In vivo studies using the Caenorhabditis elegans infection model: Toxicity and slow-killing assays Investigation of the effects of antibiofilm peptides on the innate immune system of C. elegans

Recombinant protein production: Pichia pastoris expression system, Escherichia coli expression system